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Melting of DNA (Thermal Denaturation)



abscissa: temperature, ordinate: relative UV-absorption. The transition from one conformation (double strand) to another (single strand) goes along with an increase in absorption. The Tm-value (blue line) corresponds to the temperature at which half of the molecules is single and the other half double stranded.


© Peter v. Sengbusch - b-online@botanik.uni-hamburg.de