Standard Free-Energy Changes for Glycolytic reactions at pH
7.0
Reaction (G'o values are given below each equation)
glucose <=> 2 lactate
-47 kcal. mol-1 (-197 kJ. mol-1)
ATP + H2O <=> ADP + inorganic phosphate (Pi)
-7.3 kcal. mol-1
(2 x this (-14.6 kcal. mol-1) + -32.4 kcal. mol-1
from next reaction, gives -47 kcal.mol-1 for overall)
glucose + 2 Pi + 2ADP <=> 2 lactate + 2ATP + 2H2O
-32.4 kcal. mol-1
glucose + ATP <=> G-6-P + ADP
-4.0 kcal. mol-1
G-6-P + H2O <=> glucose + Pi
-3.3 kcal. mol-1
G-6-P <=> F-6-P
0.4 kcal. mol-1
F-6-P + ATP <=> F-1,6-diP + ADP
-3.4 kcal. mol1
F-1,6-diP <=> DHAP + gly-3-P
5.73 kcal. mol-1
F-1,6-diP + H2O <=> F-6-P + Pi
-4.0 kcal. mol-1
DHAP <=> gly-3-P
1.83 kcal. mol-1
gly-3-P + NAD+ + Pi <=> 1,3-diPGA + NADH + H+
1.5 kcal. mol-1
partial reactions for above:
( RCHO + H2O + NAD+ <=> RCOOH + NADH +
H+
-10.3 kcal. mol-1 )
( RCOOH + Pi <=> RCO.OPO3 + H2O
11.8 kcal. mol-1 )
1,3-diPGA + ADP <=> 3-PGA + ADP
-4.5 kcal. mol-1
3-PGA <=> 2-PGA
1.06 kcal. mol-1
2-PGA <=> PEP + H2O
0.44 kcal. mol-1
PEP + ADP <=> pyruvate + ATP
-7.5 kcal. mol-1
pyruvate + NADH <=> lactate + NAD+
-6.0 kcal. mol-1
©Copyright 1996, Antony
Crofts, University of Illinois at Urbana-Champaign,
a-crofts@uiuc.edu
