Biophysics 354 Fall 1999
Meeting: 423, Natural History Building, 2.00 - 3.30 p.m., Tuesdays and Thursdays
Instructor: A. R. Crofts, 149 Davenport Hall (a-crofts@uiuc.edu)
TA: Masha Minchenko, (minchenko@students.uiuc.edu)
| Date | Topic | Physical chemistry discussed | Hours |
| 8/26 | Introduction | Biological energy conversion,
overview and global perspective. |
1.5 |
| 8/31 9/2 | Cellular Metabolism
Review of glycolysis;
|
Thermodynamic functions; equilibrium constant,
pH, pK, enthalpy, entropy and free energy. |
3 |
| 9/7 9/9 | Role of ATP in cell;
Review of TCC; substrate level phosphorylation |
Coupling between reactions; DGo'
for ATP
hydrolysis; redox reactions; electrode potential and relation to free energy. |
3 |
| 9/14 9/16 | Aerobic Bioenergetics
Electron transport chains;
|
Effects of ligands, H+, on redox
potential. Redox potentiometry. |
3 |
| 9/21 9/23 | Electron transport chains
as proton pumps |
Chemical potential; electrochemical
potential; membrane potential; coupling between electron and proton transport. |
3 |
| 9/28 9/30 | ATP synthesis | Proton conduction, surface potential,
local proton activity; mechanisms. |
3 |
| 10/5 10/7 | Stoichiometry | Methodology; spectrophotometry; selective electrodes.
Work, power, and potential. |
3 |
| 10/12 10/14 | Transport and Motility
Transport of metabolites; bacterial transport |
Kinetics and thermodynamics of transport. | 3 |
| 10/19 10/21 | Motility, muscle and
related systems |
Chemical and mechanical work. | 3 |
| 10/26 10/28 | Electron transfer; kinetic aspects | 1st and 2nd order kinetics, relation to equilibrium constant;
activation energy, theory of electron transfer. |
3 | 11/2 11/4 | Solar Energy Conversion
Light harvesting |
Energetics of exciton transfer;
fluorescence; Forster theory. |
3 |
| 11/9 | MID TERM EXAM | (Take home exam, no class this day) | 1.5 |
| 11/11 11/16 | Photochemical reaction
centers, bacterial RC, photosystem I |
Phototrapping; kinetics and
thermodynamics; mechanism |
3 |
| 11/18 11/23 | Photosystem II; oxygen evolution; two-electron gates | Detailed discussion of mechanism | 3 |
| 11/25 | Thanksgiving break | (No class this day) | |
| 11/30 12/2 | Photosynthetic electron transfer | Intermediate chain; bc1 complex; b6f complex | 3 |
| 12/7 12/9 | Membrane voltmeters; delayed fluorescence;
protolytic reactions |
Electrochromism; rapid pH changes. | 3 |
| 12/11 | Reading day | (No class this day) | 1.5 |
| 12/14 | FINAL EXAM DUE, 6.30 p.m. | (TAKE HOME EXAM replaces scheduled exam, 7:00-10:00 PM, Tue, Dec 14) |
Textbook
The WWW material for this course is fairly comprehensive. If you feel you need a textbook, the following is recommended:
Authors: W.A. Cramer and D.B. Knaff
Title: Energy Transduction in Biological Membranes: A Textbook of Bioenergetics
Publisher: Springer-Verlag New York/Berlin/London
Recommended
(You should have a Physical Chemistry textbook available for reference, but any good text will be sufficient).
Authors: Tinoco, Sauer and Wang
Title: Physical Chemistry, Principles and Applications in the Biological Sciences
Publishers: Prentice Hall
Author: David Walker
Title: Energy, Plants and Man
Publishers: University Science Books, Mill Valley, CA 94941