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Mammalian Reovirus Virion

as solved by Cryoelectron Microscopy and Image Reconstuction

Rotating around y axis

MPEG version (1400K)
Quicktime version (1513K)

Cropping frontal sections

MPEG version (208K)
Quicktime version (324K)

Summary

Animations of the virion of mammalian reovirus, as solved by cryo-electron microscopy and image reconstruction. Rendered as an isosurface using Iris Explorer on a Silicon Graphics workstation.

Isosurfaces are used to portray the three-dimensional structure of the virus. An isosurface is obtained by converting the subset of electron density values in the three-dimensional array that contours a chosen threshold value (isovalue) into a surface representation approximated by polygons.

Structural elements of interest include the five-fold vertices (five-fold symmetrical stars) containing lambda-2 protein and the hexameric and tetrameric flower-like projections composed of sigma-3 protein.

Created October 1994.

Acknowledgements

We gratefully acknowledge Timothy S. Baker and colleagues at the Structural Biology Group at Purdue University for supplying us with the three-dimensional cryo-electron microscopy datasets which were used to generate these images and animations.

Reference

Dryden, K.A., G. Wang, M. Yeager, M.L. Nibert, K.M. Coombs, D.B. Furlong, B.N. Fields, and T.S. Baker. 1993. Early steps in reovirus infection are associated with dramatic changes in supramolecular structure and protein conformation: analysis of virions and subviral particles by cryoelectron microscopy and image reconstruction. J. Cell Biol. 122:1023-1041.

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© 1994 Stephan Spencer. Institute for Molecular Virology/ sspencer@netconcepts.com



© 1994-1997 Stephan Spencer & Jean-Yves Sgro. Web Design By Internet Concepts LLC

 

Last Modified May 15, 1998