LON-CAPA C-Fern Educational Kits - Battle of the Sexes

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Supported, in part, by the National Science Foundation - Division of Undergraduate Education

Development of C-Fern is supported
by the National Science Foundation (NSF-DUE)

Copyright © 1997-2000
Thomas R. Warne and
Leslie G. Hickok.
All rights reserved.

C-Fern Educational Kits

Battle of the Sexes - C-Fern Sexual Differentiation Kit (15-6752)



	C-Fern Educational Materials are available from Carolina Biological Supply Company. For information please contact Carolina Biological at http://www.carolina.com or call: 800-334-5551 fax 800-222-7112.

	C-Fern Educational Kits Meet The C-Fern Sex in a Dish: The C-Fern Life Cycle Kit The Pressures of Life - Population Density and C-Fern Development Kit More Pressures of Life Kit Genetics in Action - Mendelian Genetics Kit Chemical Attraction: C-Fern Sperm Chemotaxis Accessory Kit

Students actively explore the nature of sexual differentiation in C-Fern. Although all C-Fern gametophytes develop from genetically identical spores, their development into males or hermaphrodites is environmentally controlled by a pheromone-like system. Students investigate this phenomenon experimentally by repeatedly inoculating culture dishes over a period of 7 days and analyzing the resulting sex ratios. Students formulate testable hypotheses to account for the presence and frequency of each sexual type in different aged inoculations. In addition to the wild type, this exercise uses a maleless strain (her1) which is insensitive to the pheromonal system. Materials for 30 individuals. Experiment time is 14 days.

*1 Vial Presterilized Wild Type (RNWT1) C-Fern (Ceratopteris) Spores
*1 Vial Presterilized Maleless (her1) C-Fern (Ceratopteris) Spores
*4 Bottles C-Fern Basic Medium
*40 Sterile Petri Dishes 60 x 15mm
*Sterile Water
*10 Sterile Pipets
Pen
1 Culture Dome
*10 Sterile Spore Spreaders
2 Foil Bags

15-6753. Refurbishment Set for15-6752. Includes all items with *.