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| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
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Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
| Web Journal |
| Educational Resources |
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National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
| Educational
Materials |
| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
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| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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C-Fern Educational Kits
C-Fern kits are designed for classroom use by 30 students working individually or in groups, depending upon the exercise used. All kits come complete with spores, petri dishes, C-Fern media, detailed directions for students and instructors. For predictable results in C-Fern gametophyte development, adequate lighting is necessary and can be provided by a simple fluorescent lighting fixture. A stereomicroscope and/or compound microscope is necessary for viewing the stages. Spores in all kits are provided pre-sterilized and pre-weighed in graduated vials. Students just add water, inoculate petri dishes containing C-Fern agar and maintain the dishes in Culture Domes that are a part of every kit. Cultures are grown under continuous lighting at a temperature close to 28 C inside the Culture Dome. Spore germination and gametophyte development to sexual maturity, sexual reproduction and early embryo development all take place within 14 days. Continued development of the sporophyte and the meiotic production of spores takes place within 3 months. Because each culture dish contains hundreds of individuals, large population studies of genetic segregation, competition and other plant-plant interactions are accomplished easily.
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