Other Resources

C-Fern Web Manual

• What is C-Fern (Ceratopteris)?
• Why Use C-Fern?

BASIC C-Fern NUTRIENT MEDIUM

• Preparation of agar-solidified Basic C-Fern Nutrient Medium from stock solutions
  

CULTURE CONDITIONS

• Culture Domes
• C-Fern Growth Pods
• Temperature optima and control
• Light requirements for gametophye growth
• Optimal pH of culture media for gametophyte growth
• Construct a Light Stand
  

GAMETOPHYTE CULTURE

• Spore surface sterilization
• Spore density and calculations
• Inoculation of spores
• Timing of spore germination and gametophyte development
• Synchronize spore germination and gametophyte development
• Selfing and crossing techniques

OBSERVATIONS AND ANALYSIS OF C-Fern GAMETOPHYTES

• Preparation of semi-permanent slides

SPOROPHYTE CULTURE

• Transplantation of young sporophytes into pot or terrarium culture
• Fertilization of sporophytes for optimum growth
• Cloning sporophytes - more of the same!
• Harvesting spores from mature sporophytes
• Use of Indiana and Tennessee Topsoils as potting medium for C-Fern sporophytes
• Construct a Mini-terrarium from a plastic drink bottle for your C-Fern sporophytes

SPORE MUTAGENESIS

• Ethyl Methane Sulfonate (EMS) Mutagenesis
• X-Ray Mutagenesis

SELECTED BIBLIOGRAPHY of C-Fern (Ceratopteris) publications

C-Fern INVESTIGATIONS

• Meet The C-Fern
• Sex in a Dish: The C-Fern Life Cycle Kit
• The Pressures of Life - Population Density and C-Fern Development Kit
• More Pressures of Life Kit
• Genetics in Action - Mendelian Genetics Kit
• Battle of the Sexes - C-Fern Sexual Differentiation Kit
• Chemical Attraction: C-Fern Sperm Chemotaxis Accessory Kit