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| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
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Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
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National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
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| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
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| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
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Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
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C-Fern
Sport Report
Initial characterization of this EMS-induced mutation suggests that it is highly sensitive to the pheromone, ACe. Isolates of C-Fern gametophytes typically always develop as hermaphrodites. In contrast, him1 types can spontaneously develop as males in isolate culture and there are correspondingly higher numbers of males in multispore cultures. In addition, him1 hermaphrodites tend to have much higher numbers of antheridia, especially as they age. The higher numbers of males and antheridia combine to produce cultures that have the capacity to produce many sperm that can be observed in large masses. Banks, J. A., L. Hickok and M. A. Webb. 1993. The programming of sexual phenotype in the homosporous fern, Ceratopteris richardii. Int. J. Plant Sci. 154:522-534 Banks, J. A. 1997. Sex determination in the fern Ceratopteris. Trends in Plant Science 2:175 – 179. Warne, T. R., L. G. Hickok and R. J. Scott. 1988. Characterization and genetic analysis of antheridiogen insensitive mutants in Ceratopteris richardii. Bot. J. Linn. Soc. 96: 371-379. |
