Other Resources

• INTRODUCTION

• Why Use C-Fern?

• C-Fern and the National Science Standards

• What is Ceratopteris?

• Taxonomy

• The C-Fern Life Cycle

• A Perspective of Plant Reproduction - C-Fern and teaching the concept of the Alternation of Generations
  
  

• CULTURE INSTRUCTIONS FOR C-FERN™ INVESTIGATIONS

• Culture Conditions for Growth of Gametophytes

• Culture Domes

• Temperature

• Light
  
  

• Basic C-Fern Nutrient Medium

• Preparation Of Bottled C-Fern Media

• Preparation Of Powdered Basic C-Fern Nutrient Medium

• Preparation Of Agar-Solidified Basic C-Fern Nutrient Medium From Stock Solutions
  
  

• Handling of C-Fern Spores

• Culture Inoculation With Surface-Sterilized C-Fern Spores

• Seven Good Habits for Sowing C-Fern Cultures

• Making a Spore Vial Holder

• Darkstart Method

• Dry Sowing of Presterilized C-Fern Spores

• Surface Sterilization Of C-Fern Spores

• Step by Step Procedure for Spore Sterilization

• Hints For Large Classes
  
  

• Developmental Timing of Cultures

• Observation of Cultures

• Sporophyte Culture

• Additional Information

• Equipment and Supplies Recommended for C-Fern Culture
  
  

• Appendix

  Additional Culture Instructions

• Liquid Culture of Gametophytes

• Spore Mutagenesis

• Ethyl Methane Sulfonate (Ems) Mutagenesis

• X-Ray Mutagenesis
  
  

• Controlled fertilizations: Selfing and Crossing Teschniques

• Self-Fertilization

• Cross-Fertilization
  
  

• Observations and Analysis dof Gametophytes

• Greeenhouse Culture of Sporophytes

• Transplantation

• Fertilization
  
  

• Cloning of Sporophytes

• Spore Collection
  
  

• Independent Student Research Projects

• Sport Reports

• Glossary of Terms

• Selected Bibliography

C-Fern Investigations

• Meet The C-Fern
• Sex in a Dish: The C-Fern Life Cycle Kit
• The Pressures of Life - Population Density and C-Fern Development Kit
• More Pressures of Life Kit
• Genetics in Action - Mendelian Genetics Kit
• Battle of the Sexes - C-Fern Sexual Differentiation Kit
• Chemical Attraction: C-Fern Sperm Chemotaxis Accessory Kit

Acknowledgements We thank the following individuals for contributions towards the optimization of Ceratopteris culture: Jennifer Panter, Dr. Dale Vogelien, and Stephenie Baxter.